重组人CD40蛋白(Fc Chimera Active) (ab83917)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
描述
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产品名称
重组人CD40蛋白(Fc Chimera Active)
参阅全部 CD40 蛋白酶 -
生物活性
Activity: The ED50 of ab83917 is typically 1.0-1.5 ug/ml as measured by its ability to neutralize CD40 ligand mediated proliferation of T47-D cells.
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纯度
> 95 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
Theoretical Sequence EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDT WNR ETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVL HRSC SPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLV VQQAG TNKTDVVCGPQDRLRRSSNTKVDKKVEPKSCDKTHTCPPCPAPELLGG PSVFL FPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTK PREEQ YNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP REPQVY TLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPV LDSDG SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
氨基酸
21 to 193 -
额外的序列信息
Fused with the Fc region of Human IgG1 at the C-terminus.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab83917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: PBS, 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
常规信息
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别名
- AI326936
- B cell associated molecule CD40
- B cell surface antigen CD40
see all -
功能
Receptor for TNFSF5/CD40LG. -
组织特异性
B-cells and in primary carcinomas. -
疾病相关
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
序列相似性
Contains 4 TNFR-Cys repeats. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
图片
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Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein. -
1D SDS-PAGE of ab83917 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83917
Lane 2: ab83917 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83917 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie. -
Anti-CD40 antibody (ab13545) at 1/1000 dilution +
Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab83917 尚未被引用在任何文献中。