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I am using your Sag 1 antigen code ab68110 in an ELISA where I am hoping to bind it to the plate by passive adsorption in carbonate buffer at ph9.6.
I notice the storage buffer contains urea, can you advise me what the function of this is in the storage buffer and therefor what effect diluting it out in order to coat the plate may have on the properties of the protein. Can you also tell me if urea is detrimental in an ELISA and if we should be dialysing it out
Asked on Oct 30 2013
Urea is very strong protein denaturant which unfold proteins for greater solubility and stability. Some proteins precipitates in buffers so urea is added to increase the solubility.
There are many open access publication related to your question for further reading e.g.
This protein was tested in ELISA so it should work in ELISA experiments. The optimal dilution however should be determined by the end user. In experiments further dilution of protein should not be having any effect on the properties for the protein. Please use it without hesitation.
Padamjeet Singh Abcam Scientific Support
回复于 Oct 30 2013