重组小鼠CD3蛋白(Fc Chimera) (ab220543)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
描述
-
产品名称
重组小鼠CD3蛋白(Fc Chimera)
参阅全部 CD3 蛋白酶 -
生物活性
Immobilized ab220543 at 5 μg/mL (100 μL/well) can bind CD3e Monoclonal Antibody, Biotin with a linear range of 5-156 ng/mL (QC tested).
-
纯度
> 95 % SDS-PAGE.
Protein A purified. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
-
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Mouse -
序列
DAENIEYKVSISGTSVELTCPLDSDENLKWEKNGQELPQKHDKHLVLQDF SEVEDSGYYVCYTPASNKNTYLYLKARVCEYCVEVD -
预测分子量
36 kDa including tags -
分子量信息
The protein migrates as 40-43 kDa under reducing condition. -
氨基酸
23 to 108 -
标签
Fc tag C-Terminus -
额外的序列信息
Fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 5410-5413 kDa under reducing (R) condition (SDS-PAGE).
-
技术指标
Our Abpromise guarantee covers the use of ab220543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
ELISA
SDS-PAGE
-
形式
Lyophilized -
补充说明
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, 5% Trehalose, Glycine, Sodium chloride, L-ArginineThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab220543 尚未被引用在任何文献中。