概述

描述

  • 性质
    Recombinant
  • 来源
    Saccharomyces cerevisiae
  • 氨基酸序列
    • Accession
    • 分子量
      57 kDa
    • 氨基酸
      1 to 568

相关产品

技术指标

Our Abpromise guarantee covers the use of ab119881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    Western blot

    SDS-PAGE

    Electron Microscopy

  • 纯度
    > 90 % SDS-PAGE.
    ab119881 is purified by ultracentifugation.
  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Constituent: 99% PBS

  • 复溶
    Reconstitute in PBS

常规信息

  • 别名
    • L1
    • HPV18 major capsid protein L1
    • Human papilloma virus type 18 major capsid protein L1
    • Human papillomavirus type 18 L1
    • Human papillomavirus type 18 major capsid protein L1
    • Major capsid protein L1
    see all
  • 相关性
    L1 is a major capsid protein of type 18 human papilloma virus. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
  • 细胞定位
    Virion

图片

  • SDS-PAGE analysis of ab119881.

    Lane 1. ab119881, 3 µg/lane
    Lane 2. MWt marker 11.0, 17.0, 26.0, 34.0, 43.0, 55.0, 72.0, 95.0, 130.0, 170.0 kDa.
  • ab119881 viewed by electron microscopy.

文献

ab119881 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

The protein can be reconstituted in 1X PBS.

No special protocol is needed for SDS-PAGE any general SDS-PAGE protocol will work. Please mix the protein with 1:1 loading buffer and boil for 5-10 minutes before loading into gel.

Try loading 5-10ug of protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

The sequence is the 507aa GenBank gb|AAQ92369 sequence.

http://www.ncbi.nlm.nih.gov/protein/AAQ92369.1

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Answer

Thank you for providing that information.

I am sorry for the misunderstanding in this case. I am sorry to hear that the customer has not received the expected products in one of the vials. I would be happy to send a replacement to you if you would be able to provide me with the order number on which the 4 vials of ab119881 was ordered.

I have found4 separateorders of the following dates from you:



Are these the orders concerned?

Do you know which order the problem vial corresponds to?

If I arrange for a replacement vial of ab119881 to be sent could you please confirm the address I have for you is correct:
xxxxxxxxxxxxxxxxxxxx




Many thanks for your cooperation. I look forward to receiving your reply.

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Answer

Thank you for your enquiry and your interest in our products.

Please find the information regarding ab119881 below:

Question 1.)

If you dissolve the HPV18L1 protein (50 micrograms) in 50 microliters of distilled water (the storage buffer is PBS) the concentration of HPV18L1 protein will be 1 mg/ml. If you need more diluted protein, please dilute it with PBS further.

Question 2.)

The theoretical molecular weight of HPV18L1 protein is 56.5kDa but the actual weight could be slightly different. For loading on SDS PAGE (minigel) you need to prepare the dissolved HPV18L1 protein (1mg/ml) as follows:

Prepare 90 microliters of Protein Sample or Loading Buffer x1 (you can prepare it yourself or purchase it ready-made) and add 10 microliters of the dissolved HPV18L1 protein (1mg/ml). Boil the mix 10 min and load on the gel 10 microliters per track of the cooled mix.

Question 3.)

If you prepare the HPV18L1 protein as explained above you will see 1 microgram per band on SDS PAGE after staining of the gel with Coomassie Blue.

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for contacting us.

I am sorry but I am a little unsure of what the customer has asked for. Has he found no product in the vial of ab119881 received?

The HPV18 L1 protein (ab119881) is the recombinantly expressed, full length HPV18 L1 protein which has been lypholised. The protein needs to be reconsituted prior to use, it is provided as a solid and may not be visible in the vial itself. But please be assured that 50 ug of protein is provided in each vial which can be reconsituted to the concentration of your choice using PBS.

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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