存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab48811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
功能Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation.
组织特异性Specifically expressed in osteoblasts.
疾病相关Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies.
序列相似性Contains 1 Runt domain.
结构域A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites.
翻译后修饰Phosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340.
- Information by UniProt
- Acute myeloid leukemia 3 protein antibody
- Alpha subunit 1 antibody
- AML3 antibody
Lane 2 : Anti-RUNX2 antibody (ab48811) at 1.25 µg/ml
Lane 1 : MARKER
Lane 2 : Jurkat cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Zhang Y et al. Vitamin K2 promotes mesenchymal stem cell differentiation by inhibiting miR-133a expression. Mol Med Rep 15:2473-2480 (2017). WB ; Human . Read more (PubMed: 28447758) »
- Rodriguez KJ et al. Regulation of valvular interstitial cell phenotype and function by hyaluronic acid in 2-D and 3-D culture environments. Matrix Biol 30:70-82 (2011). WB ; Pig . Read more (PubMed: 20884350) »