Application
Western blot
Sample
Human cytomegalovirus Cell lysate - whole cell (HCMV-infected human foreskin fibroblasts)
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Loading amount
500000 cells
Treatment
1 µM Shield-1
Specification
HCMV-infected human foreskin fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/20000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti rabbit (Jackson ImmunoResearch)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Mock-infected cells
Exposure
15 second(s)
Bands
Specific: 220-250 kDa
Positive control
HCMV-infected cells harvested at different times post infection (HCMV infection will increase the protein level of RNAP II, including pSer2-CTD phosphor-isoform)
Additional data
Additional Notes
HFFs were infected with ADddUL79 at an MOI of 3 in the presence or absence of 1 mM Shield-1 (Shld1). Cells were harvested at different times post infection and protein accumulation was analyzed by immunoblot analysis with antibodies recognizing RNAP II pSer-2 CTD
PubMed ID
25166009: View
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提交于 Jun 16 2015