Anti-RBP1抗体(ab104452)
Key features and details
- Goat polyclonal to RBP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RBP1抗体
参阅全部 RBP1 一抗 -
描述
山羊多克隆抗体to RBP1 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide:
VEGVVCKQVFKKVQ
conjugated to KLH, corresponding to C terminal amino acids 122-135 of Human RBP1. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104452于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 16 kDa.
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IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 16 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Intracellular transport of retinol. -
组织特异性
Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver. -
序列相似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
结构域
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5947 Human
- Entrez Gene: 19659 Mouse
- Omim: 180260 Human
- SwissProt: P09455 Human
- SwissProt: Q00915 Mouse
- Unigene: 529571 Human
- Unigene: 279741 Mouse
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别名
- Cellular retinol binding protein antibody
- Cellular retinol-binding protein antibody
- Cellular retinol-binding protein I antibody
see all
图片
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ab104452, at 5 µg/ml, staining RBP1 in formalin-fixed, paraffin-embedded Human Liver by Immunohistochemistry, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab104452, at 5 µg/ml, staining RBP1 in formalin-fixed, paraffin-embedded Human Pancreas by Immunohistochemistry, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Anti-RBP1 antibody (ab104452) at 0.1 µg/ml + NIH 3T3 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 16 kDa
Primary incubation was 1 hour. -
ab104452 staining mouse liver sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab104452 at 1/100 in TBS/BSA/azide for 2h at 21°C. A biotinylated rabbit anti-goat polyclonal antibody at 1/250 was used as the secondary antibody. Red arrowheads indicate strongly stained Kupffer cells.
数据表及文件
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Datasheet download
文献 (0)
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