Proteinase K Antigen Retrieval Solution (ab64220)
概述
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产品名称
Proteinase K Antigen Retrieval Solution -
经测试应用
适用于: IHC-P, In situ hybridizationmore details -
常规说明
ab64220 is a single-component, ready-to-use proteinase K solution for use in protease-induced antigen retrieval during IHC (PIER) and pre-treatment for in situ hybridization.
Staining Protocol:
- IHC procedure: Incubate tissue section for 5 to 7 minutes at room temperature; Rinse and proceed with immunostaining.
- General ISH procedure: Incubate tissue section for 15 minutes at 37oC; Rinse and proceed with hybridization.
Other products for IHC
Other enzymes for antigen retrieval include: Pepsin Solution ab64201 and Trypsin Antigen Retrieval Kit ab259256.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
性能
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形式
Liquid -
存放说明
Store at -20°C. Stable for 12 months at -20°C -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: Tris buffer, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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相关性
Proteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name. Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes. -
别名
- Endopeptidase K
- Tritirachium alkaline proteinase
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64220于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent dilution.
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In situ hybridization |
Use at an assay dependent dilution.
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说明 |
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IHC-P
Use at an assay dependent dilution. |
In situ hybridization
Use at an assay dependent dilution. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab64220 被引用在 24 文献中.
- Yurtal Z et al. The Matrix Metalloproteinase Inhibitor Batimastat Reduces Epidural Fibrosis After Laminectomy in Rats. Turk Neurosurg 33:162-170 (2023). PubMed: 36482856
- Yoshii D et al. An autopsy case of adult-onset neuronal intranuclear inclusion disease with perivascular preservation in cerebral white matter. Neuropathology 42:66-73 (2022). PubMed: 34954850
- Ou Z et al. Myeloid Poldip2 Contributes to the Development of Pulmonary Inflammation by Regulating Neutrophil Adhesion in a Murine Model of Acute Respiratory Distress Syndrome. J Am Heart Assoc 11:e025181 (2022). PubMed: 35535614
- Terzi F et al. Effects of tocilizumab and dexamethasone on the downregulation of proinflammatory cytokines and upregulation of antioxidants in the lungs in oleic acid-induced ARDS. Respir Res 23:249 (2022). PubMed: 36115998
- Mold MJ & Exley C Aluminium co-localises with Biondi ring tangles in Parkinson's disease and epilepsy. Sci Rep 12:1465 (2022). PubMed: 35087154