Anti-Proteasome subunit beta type 2抗体[MCP165] (ab22650)


  • 产品名称
    Anti-Proteasome subunit beta type 2抗体[MCP165]
    参阅全部 Proteasome subunit beta type 2 一抗
  • 描述
    小鼠单克隆抗体[MCP165] to Proteasome subunit beta type 2
  • 宿主
  • 经测试应用
    适用于: Flow Cyt, IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length protein corresponding to Human Proteasome subunit beta type 2.
    Database link: P49721

  • 阳性对照
    • HeLa cell lysate



Our Abpromise guarantee covers the use of ab22650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 28 kDa.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
    • 序列相似性
      Belongs to the peptidase T1B family.
    • 细胞定位
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HC7 I antibody
      • Macropain subunit C7 I antibody
      • Macropain subunit C7-I antibody
      • Multicatalytic endopeptidase complex subunit C7 1 antibody
      • Multicatalytic endopeptidase complex subunit C7 I antibody
      • Multicatalytic endopeptidase complex subunit C7-I antibody
      • Proteasome (prosome, macropain) subunit beta type 2 antibody
      • Proteasome beta 2 subunit antibody
      • Proteasome component C7 I antibody
      • Proteasome component C7-I antibody
      • Proteasome subunit beta type-2 antibody
      • PSB2_HUMAN antibody
      • Psmb2 antibody
      see all


    • Lane 1 : Protein Marker IV
      Lanes 2-7 : Anti-Proteasome subunit beta type 2 antibody [MCP165] (ab22650) at 1/1000 dilution

      Lane 1 : Protein Marker IV
      Lanes 2 & 4 & 6 : Protein extract from ciliary body of diseased human donor at 2 µg
      Lanes 3 & 5 & 7 : Protein extract from ciliary body of normal human donor at 2 µg

      Lanes 2-7 : HRP conjugated goat anti mouse antibody

      Observed band size: 27 kDa (why is the actual band size different from the predicted?)

      See Abreview

    • IHC image of ab22650 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22650, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Overlay histogram showing HeLa cells stained with ab22650 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22650, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


    This product has been referenced in:
    • Leite NC  et al. Protein malnutrition potentiates the amplifying pathway of insulin secretion in adult obese mice. Sci Rep 6:33464 (2016). WB ; Mouse . Read more (PubMed: 27633083) »
    • Ren H  et al. Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice. Sci Rep 5:15730 (2015). Read more (PubMed: 26508670) »

    See all 4 Publications for this product


    I can confirm that this particular antibody is sourced externally from a collaborator. Unfortunately we are not able to release the immunogen sequence as they consider this proprietary information.

    They have confirmed that this antibody r...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Tissue lysate - whole (ciliary body)
    Loading amount
    2 µg
    ciliary body
    Blocking step
    BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%

    Dr. Matthias Zenkel

    Verified customer

    提交于 May 04 2007