Application
ELISA
Sample
Human Purified protein (Prostate Specific Antigen from human semen(Purity)
Specification
Prostate Specific Antigen from human semen(Purity
Type
Sandwich (Capture)
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: MES
Dilution
1/40
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
*Protocol
1. 480 µg carboxyl terminated beads with 2 mM EDC, 5 mM NHS in 500 µl MES buffer r.t 30 min and washing
2. anti-PSA from Mouse (ab403, abcam) 4 µg in 40 µl MES buffer r.t overnight and washing
3. 60 µg antibody conjugated beads with PSA antigen (P3235, Sigma) r.t 30min in 500 µl whole human serum
4. T-TBS washing 2 times
5. Antigen captured beads + 1.2 µl matrix solution(10 mg/ml sinapinic acid in 50:50:0.1 (v/v/v) acetonitrile, water, trifluoroacetic acid containing 10 fmol/µl cytochrome C as internal standard)
6. Plating and MALDI-TOF analysis
Ref. Chem. Commun., 2014, 50, 10066-10069
PubMed ID
25036285: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Chang Su Jeon
Verified customer
提交于 Jul 27 2015