Application
Western blot
Sample
Human Cell lysate - whole cell (vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
UV irradiation 20 J/m2 (30 mins recovery)
Specification
vascular smooth muscle cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST, 5% milk
Concentration
2 µg/ml
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 120 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Feb 06 2014