Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Adult mouse testis)
Specification
Adult mouse testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/500
Additional data
Additional Notes
Plzf should show nuclear expression, however, this antibody detected cytoplasmic expression. I think it might be detecting a different protein?
Abcam response
Thank you for sharing this data with us. ab39354 has been successfully used on PFA perfusion fixed paraffin embedded mouse tissue sections (see PubMed: 21228182). From this data, however, it is clear that not all protocols will be equally suitable for this technique. Abcam would welcome feedback from other customers using this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. 煜欽 林
Verified customer
提交于 Nov 20 2012