Anti-Plzf抗体(ab39354)
Key features and details
- Rabbit polyclonal to Plzf
- Suitable for: WB
- Reacts with: Human, Zebrafish
- Isotype: IgG
概述
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产品名称
Anti-Plzf抗体
参阅全部 Plzf 一抗 -
描述
兔多克隆抗体to Plzf -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Zebrafish
预测可用于: Mouse, Rat, Cow -
免疫原
Synthetic peptide corresponding to Human Plzf aa 650 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab39353) -
阳性对照
- This antibody gave a positive signal in HL60 and Ramos whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39354于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human Plzf peptide (ab39353).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human Plzf peptide (ab39353). |
靶标
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功能
Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
组织特异性
Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Skeletal defects, genital hypoplasia, and mental retardation
A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 9 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 534401 Cow
- Entrez Gene: 7704 Human
- Entrez Gene: 235320 Mouse
- Entrez Gene: 353227 Rat
- Entrez Gene: 323269 Zebrafish
- Omim: 176797 Human
- SwissProt: Q05516 Human
- SwissProt: Q802Y8 Zebrafish
see all -
别名
- AI467657 antibody
- lu antibody
- PLZF antibody
see all
图片
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All lanes : Anti-Plzf antibody (ab39354) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutes -
All lanes : Anti-Plzf antibody (ab39354) at 1.25 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab39354 被引用在 16 文献中.
- Seth A et al. Gene dosage changes in KCTD13 result in penile and testicular anomalies via diminished androgen receptor function. FASEB J 36:e22567 (2022). PubMed: 36196997
- Bode MF et al. Cell type-specific roles of PAR1 in Coxsackievirus B3 infection. Sci Rep 11:14264 (2021). PubMed: 34253819
- Li SH et al. Metabotropic Glutamate Receptor 2/3 Activation Improves Motor Performance and Reduces Pathology in Heterozygous zQ175 Huntington Disease Mice. J Pharmacol Exp Ther 379:74-84 (2021). PubMed: 34330748
- Guo Y et al. Suppressive role of E3 ubiquitin ligase FBW7 in type I diabetes in non-obese diabetic mice through mediation of ubiquitination of EZH2. Cell Death Discov 7:361 (2021). PubMed: 34802056
- Lei JH et al. Cytotoxicity of nonylphenol on spermatogonial stem cells via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway. World J Stem Cells 12:500-513 (2020). PubMed: 32742567