Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: universal decloaker
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/150
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: DAKO K4003 goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
PubMed ID
n/a: View
Additional Notes
For antigen retrieval, Biocare UD1000M universal decloaker soultion was used. After deparaffinized and rehydration, tissue sections were heated to 125'C for 30 sec in the presence of 1x universal decloaker solution inside a Decloaking chamber (kind of digital control pressure cooker). After rising with with distilled water and PBS, the sections were ready for further blocking, antibody binding and detection of antigen of interest.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 27 2012