Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Sample buffer: PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA in PBST
Dilution
1/300
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor© 488 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
This dot plot shows the negative population in white and the stained population in green. Green gating is based on the isotype control (<1%). Histogram will not be as clear as the dot plot submitted.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 18 2015