Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus and Liver)
Gel Running Conditions
Reduced Denaturing (8% in TGS)
Loading amount
30 µg
Specification
Hippocampus and Liver
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Cor Block Buffer
Dilution
1/1200
Secondary antibody
Dilution
1/18000
Name
Non-Abcam antibody was used: Li-Cor IRDye Donkey anti Mouse 680LT
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Detection
Detection method
IR
Negative control
Whole Liver
Exposure
5 minute(s) and 30 second(s)
Bands
Specific: ~95 kDa Non-specific: 75 kDa
Positive control
Whole Hippocampi
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Grant Corbett
Verified customer
提交于 Jul 17 2014