概述

  • 产品名称
  • 描述
    兔多克隆抗体to PIWIL2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-Fr, WB, Flow Cyt, ICC/IF, IP, Electron Microscopymore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse MILI/ HILI.

    参阅Abcam的专有抗源政策 (Peptide available as ab27821.)

  • 阳性对照
    • Mouse Testis Whole Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab36764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 20534472
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).Can be blocked with Mouse PIWIL2 peptide (ab27821).
Flow Cyt Use at an assay dependent concentration. PubMed: 20460113

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration. PubMed: 20460113
IP Use at an assay dependent concentration. PubMed: 20460113
Electron Microscopy Use at an assay dependent concentration. PubMed: 20011505

靶标

  • 功能
    Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
  • 组织特异性
    Expressed in adult testis and in most tumors.
  • 序列相似性
    Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 翻译后修饰
    Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
  • 细胞定位
    Cytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancer/testis antigen 80 antibody
    • CT80 antibody
    • Hiwi like antibody
    • MILI antibody
    • Miwi like antibody
    • Piwi like 2 antibody
    • Piwi-like protein 2 antibody
    • Piwil2 antibody
    • PIWL2_HUMAN antibody
    see all

图片

  • Anti-PIWIL2 antibody (ab36764) at 1 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 110 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

  • ab36764 staining PIWIL2 in murine testis tissue by Immunohistochemistry (Frozen sections). Chromatin was stained with DAPI.
  • Testes section from adult mice were immunostained with anti-PIWIL2 (MILI) antibody ab36764. Nuclei were stained with DAPI.  Testes were fixed in 4% PFA at 4°C for 3 hours and overnight respectively, dehydrated in 30% sucrose overnight, frozen in dry ice/95% ethanol, and sectioned at –20°C.

文献

This product has been referenced in:
  • Feng D  et al. Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis. Oncotarget 7:64575-64588 (2016). IP ; Human . Read more (PubMed: 27602489) »
  • Fu Q  et al. Mutations in the MOV10L1 ATP Hydrolysis Motif Cause piRNA Biogenesis Failure and Male Sterility in Mice. Biol Reprod 95:103 (2016). Read more (PubMed: 27655786) »
See all 10 Publications for this product

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

The Western Blot image was obtained using 1 µg/ml of ab36764.

The IHC-Fr image comes from a publication : http://www.ncbi.nlm.nih.gov/pubmed/20534472?dopt=Abstract. Unfortunately there is no information about the concentration used. I would suggest to contact the author. Otherwise I would recommendto use ab36764 in IHC-Fr ata concentration of 10 µg/ml as a starting pointand to optimise the results around this value.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Testis)
Loading amount
30 µg
Specification
Testis
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

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提交于 Feb 04 2008

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