Application
Western blot
Sample
Mouse Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Sciatic nerve
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk (TBS-tween)
Dilution
1/1000
Secondary antibody
Dilution
1/6000
Name
Non-Abcam antibody was used: Goat anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
8 minute(s) and 40 second(s)
Bands
Specific: 25 kDa Non-specific: 33 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 22 2016