Anti-PDLIM5/ENH抗体(ab85967)
Key features and details
- Rabbit polyclonal to PDLIM5/ENH
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PDLIM5/ENH抗体
参阅全部 PDLIM5/ENH 一抗 -
描述
兔多克隆抗体to PDLIM5/ENH -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human PDLIM5/ENH aa 500-600 (C terminal).
Database link: Q96HC4 -
阳性对照
- WB: HeLa whole cell lysate. IP: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85967于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 64 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses. -
组织特异性
Heart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression. -
序列相似性
Contains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse > synaptosome. Cytoplasm. Detected both at presynaptic and postsynaptic sites. - Information by UniProt
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数据库链接
- Entrez Gene: 503621 Cow
- Entrez Gene: 10611 Human
- Entrez Gene: 56376 Mouse
- Entrez Gene: 64353 Rat
- Omim: 605904 Human
- SwissProt: Q96HC4 Human
- SwissProt: Q8CI51 Mouse
- SwissProt: Q62920 Rat
see all -
别名
- ENH antibody
- ENH1 antibody
- Enigma homolog antibody
see all
图片
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All lanes : Anti-PDLIM5/ENH antibody (ab85967) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 64 kDa
Exposure time: 30 seconds -
1mg HeLa whole cell lysate was immunoprecipitated using ab85967 at 3µg. 20% of IP was loaded/lane and PDLIM5/ENH was detected with ab85967 at 1µg/ml (lane 1) or a control IgG (lane 2).
Detection: chemiluminescence with exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab85967 尚未被引用在任何文献中。