参阅全部 PDE3B 一抗
经测试应用适用于: WB, IPmore details
预测可用于: Horse, Chicken, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to a region between residue 1062 and 1112 of human PDE3B. (NP_000913.2)
- HeLa whole cell lysate
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab99290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 124 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
功能Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism.
组织特异性Abundant in adipose tissues.
序列相似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.
- Information by UniProt
- 5''-cyclic phosphodiesterase B antibody
- Adipocyte cAMP phosphodiesterase cGMP inhibited antibody
- AI847709 antibody
All lanes : Anti-PDE3B antibody (ab99290) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 124 kDa
Lane 1: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody recognising an upstream epitope of PDE3B.
Lane 2: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab99290 at 3 µg/mg lysate for IP.
Lane 3 : Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab99290 at 1 µg/ml. Developed using the ECL technique with an exposure time of 10 seconds.
ab99290 has not yet been referenced specifically in any publications.