Application
Western blot
Sample
Human Cell lysate - whole cell (786-0, a renal cell carcinoma cell line)
Loading amount
50 µg
Specification
786-0, a renal cell carcinoma cell line
Gel Running Conditions
Reduced Denaturing (4-12% gradient gel)
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% Tween-20 in Odyssey blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse Alexa Fluor 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/3000
Detection
Detection method
Fluorescence
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 50 kDa Non-specific: many kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 26 2013