Anti-PAK4抗体(ab17974)
Key features and details
- Rabbit polyclonal to PAK4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PAK4抗体
参阅全部 PAK4 一抗 -
描述
兔多克隆抗体to PAK4 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Guinea pig, Dog, Pig, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human PAK4 aa 1-100.
Database link: O96013 -
阳性对照
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17974于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 64 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 64 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2. -
组织特异性
Highest expression in prostate, testis and colon. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated on serine residues when activated by CDC42/p21.
Phosphorylated on tyrosine residues upon stimulation of FGFR2. - Information by UniProt
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数据库链接
- Entrez Gene: 484513 Dog
- Entrez Gene: 100736193 Guinea pig
- Entrez Gene: 10298 Human
- Entrez Gene: 70584 Mouse
- Entrez Gene: 100627936 Pig
- Entrez Gene: 100341934 Rabbit
- Omim: 605451 Human
- SwissProt: O96013 Human
see all -
别名
- KIAA1142 antibody
- p21 activated kinase 4 antibody
- p21 Cdc42/Rac1-actiated kinase 4 antibody
see all
图片
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All lanes : Anti-PAK4 antibody (ab17974) at 0.04 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 64 kDa
Exposure time: 3 minutes -
PAK4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab17974 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab17974 at 0.4 µg/ml.
Lane 1: ab17974 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab17974 尚未被引用在任何文献中。