Anti-P2X7抗体(ab93354)
Key features and details
- Goat polyclonal to P2X7
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-P2X7抗体
参阅全部 P2X7 一抗 -
描述
山羊多克隆抗体to P2X7 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Guinea pig, Dog -
免疫原
Synthetic peptide corresponding to Human P2X7 aa 13-26 (N terminal) (Cysteine residue).
Sequence:YETNKVTRIQSMNY
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阳性对照
- WB: Human brain frontal cortex lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93354于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37, 70 kDa (predicted molecular weight: 69 kDa).
1 hour primary incubation is recommended for this product. |
IHC-P |
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37, 70 kDa (predicted molecular weight: 69 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. -
组织特异性
Widely expressed with highest levels in brain and immune tissues. -
序列相似性
Belongs to the P2X receptor family. -
翻译后修饰
Phosphorylation results in its inactivation.
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5027 Human
- Entrez Gene: 18439 Mouse
- Entrez Gene: 29665 Rat
- Omim: 602566 Human
- SwissProt: Q99572 Human
- SwissProt: Q9Z1M0 Mouse
- SwissProt: Q64663 Rat
- Unigene: 729169 Human
see all -
别名
- ATP receptor antibody
- P2rx7 antibody
- P2RX7_HUMAN antibody
see all
图片
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All lanes : Anti-P2X7 antibody (ab93354) at 0.3 µg/ml
Lane 1 : Human brain frontal cortex lysate
Lane 2 : Human brain frontal cortex lysate with Immunising peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 37,70 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
数据表及文件
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Datasheet download
文献 (7)
ab93354 被引用在 7 文献中.
- Han Y et al. P2X7 receptor involved in antitumor activity of atractylenolide I in human cervical cancer cells. Purinergic Signal 19:145-153 (2023). PubMed: 35235139
- Stähler T et al. Development of Antibody and Nanobody Tools for P2X7. Methods Mol Biol 2510:99-127 (2022). PubMed: 35776322
- Zhang Q et al. The ATP-P2X7 Signaling Pathway Participates in the Regulation of Slit1 Expression in Satellite Glial Cells. Front Cell Neurosci 13:420 (2019). PubMed: 31607866
- Asif A et al. Role of purinergic receptors in hepatobiliary carcinoma in Pakistani population: an approach towards proinflammatory role of P2X4 and P2X7 receptors. Purinergic Signal 15:367-374 (2019). PubMed: 31401785
- Cabahug-Zuckerman P et al. Potential role for a specialized ß3 integrin-based structure on osteocyte processes in bone mechanosensation. J Orthop Res 36:642-652 (2018). PubMed: 29087614
- Liu J et al. Hypermethylation downregulates P2X7receptor expression in astrocytoma. Oncol Lett 14:7699-7704 (2017). PubMed: 29344216
- Zhang PA et al. Neonatal Maternal Deprivation Enhances Presynaptic P2X7 Receptor Transmission in Insular Cortex in an Adult Rat Model of Visceral Hypersensitivity. CNS Neurosci Ther 23:145-154 (2017). PubMed: 27976523