Anti-Otx1抗体(ab25985)
Key features and details
- Rabbit polyclonal to Otx1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-Otx1抗体
参阅全部 Otx1 一抗 -
描述
兔多克隆抗体to Otx1 -
宿主
Rabbit -
特异性
We have not been able to test Rabbit polyclonal to Otx1 (ab25985) in Western Blot due to difficulties in accessing the appropriate lysates. We would appreciate any feedback on this antibody. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chimpanzee, Gorilla, Catfish -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse Otx1.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25985于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (3) |
Use at an assay dependent concentration.
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 37 kDa).
This application has only been tested on recombinant protein. |
说明 |
---|
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 37 kDa). This application has only been tested on recombinant protein. |
靶标
-
功能
Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. -
组织特异性
Expressed in brain. -
序列相似性
Belongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain. -
发展阶段
Embryo. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 5013 Human
- Entrez Gene: 18423 Mouse
- Entrez Gene: 25646 Rat
- Omim: 600036 Human
- SwissProt: P32242 Human
- SwissProt: Q53TG6 Human
- SwissProt: P80205 Mouse
- SwissProt: Q5SS54 Mouse
see all -
别名
- FLJ38361 antibody
- Homeobox protein OTX 1 antibody
- Homeobox protein OTX1 antibody
see all
图片
-
Anti-Otx1 antibody (ab25985) at 1 µg/ml + OTX1 (Human) - Recombinant Protein at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25985 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Ab25985 recognizes the full length tagged recombinant protein which has an expected molecular weight of 75 kDa.
-
IHC-P image of Otx1 antibody (ab25985) on E11.5 mouse embryo sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using EDTA. These sections were then blocked in serum for 1 hour, before the Otx1 antibody was added at 1:100 dilution and left overnight at 4oC.
数据表及文件
-
SDS download
-
Datasheet download
文献 (7)
ab25985 被引用在 7 文献中.
- Zhou L et al. OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway. Oncol Rep 48:N/A (2022). PubMed: 36177903
- Jiang L et al. Orthodenticle homeobox OTX1 is a potential prognostic biomarker for bladder cancer. Bioengineered 12:6559-6571 (2021). PubMed: 34559577
- Chen S et al. MicroRNA-4516 suppresses pancreatic cancer development via negatively regulating orthodenticle homeobox 1. Int J Biol Sci 16:2159-2169 (2020). PubMed: 32549762
- Elwakeel E et al. Phenotypic Plasticity of Fibroblasts during Mammary Carcinoma Development. Int J Mol Sci 20:N/A (2019). PubMed: 31505876
- Xu PC et al. Myeloperoxidase influences the complement regulatory function of modified C-reactive protein. Innate Immun : (2013). PubMed: 24189124
- Saito T et al. Neocortical Layer Formation of Human Developing Brains and Lissencephalies: Consideration of Layer-Specific Marker Expression. Cereb Cortex : (2010). PubMed: 20624841
- Hadjivassiliou G et al. The application of cortical layer markers in the evaluation of cortical dysplasias in epilepsy. Acta Neuropathol : (2010). IHC-P ; Human . PubMed: 20411268