参阅全部 Osteopontin 一抗
经测试应用适用于: ICC/IF, IHC-P, WBmore details
种属反应性与反应: Mouse, Rat, Human
- This antibody gave a positive signal in the following lysates: Rat Skeletal Muscle Tissue Rat Brain Tissue HL60 Whole Cell Mouse Brain Tissue Rat Liver Tissue (data not shown) Mouse Liver Tissue (data not shown) Human Liver Tissue (data not shown)
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab63856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).|
功能Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
组织特异性Bone. Found in plasma.
序列相似性Belongs to the osteopontin family.
翻译后修饰Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- BNSP antibody
- Bone sialoprotein 1 antibody
- BSP I antibody
IHC image of Osteopontin staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63856, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ICC/IF image of ab63856 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63856, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Osteopontin antibody (ab63856) at 1 µg/ml
Lane 1 : Skeletal Muscle (Rat) Tissue Lysate - normal tissue (ab29376)
Lane 2 : Brain (Rat) Tissue Lysate - normal tissue
Lane 3 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate
Lane 4 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: 100 kDa, 42 kDa, 56 kDa, 88 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Yan Y et al. Effect of Thyrotropin on Osteopontin, Integrin avß3, and VCAM-1 in the Endothelium via Activation of Akt. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27657042) »
- Koczorowska MM et al. Fibroblast activation protein-a, a stromal cell surface protease, shapes key features of cancer associated fibroblasts through proteome and degradome alterations. Mol Oncol 10:40-58 (2016). Read more (PubMed: 26304112) »