Anti-Osteocalcin抗体[OCG3] (ab13420)


  • 产品名称
    参阅全部 Osteocalcin 一抗
  • 描述
    小鼠单克隆抗体[OCG3] to Osteocalcin
  • 宿主
  • 经测试应用
    适用于: WB, IHC-Fr, IHC-P, Sandwich ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Sheep, Rabbit, Goat, Chicken, Cow, Dog, Human, Pig
    不与反应: Mouse
  • 免疫原

    Full length protein corresponding to Cow Osteocalcin.
    Database link: P02820

  • 表位
    Residues 21-31
  • 阳性对照
    • Normal bovine bone tissue
  • 常规说明

    Though we have found ab13420 does not react with mouse sample in our lab, there is a report from a reviewer that got positive result with mouse sample in IHC-Fr. However, this information needs to be confirmed further.


    Although this antibody does work in IHC-P, we have found that ab13418 typically gives a more robust signal. Therefore, if you plan on using this antibody in IHC-P, we recommend ab13418 as an alternative option.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 



Our Abpromise guarantee covers the use of ab13420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12 kDa.

Use under reducing or non-reducing conditions.

IHC-Fr Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Enzymatic retrieval with Proteinase K is recommended.
Sandwich ELISA Use a concentration of 1 - 10 µg/ml.

For use in sandwich ELISA with ab13421

ICC/IF Use at an assay dependent concentration. PubMed: 21396708


  • 功能
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 序列相似性
    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • 翻译后修饰
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all


  • Detection of human osteocalcin by ab13420
  • Image from Strauß S et al., PloS One. 2012;7(12):14. Fig 8.; doi: 10.1371/journal.pone.0051264


This product has been referenced in:
  • Zhou D  et al. Valproic acid prevents glucocorticoid-induced osteonecrosis of the femoral head of rats. Int J Mol Med 41:3433-3447 (2018). Read more (PubMed: 29512684) »
  • Zhang J  et al. Repair of Critical-Sized Mandible Defects in Aged Rat Using Hypoxia Preconditioned BMSCs with Up-regulation of Hif-1a. Int J Biol Sci 14:449-460 (2018). Read more (PubMed: 29725266) »

See all 43 Publications for this product


Human Cultured Cells (PULP)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Kyungjoo Seong

Verified customer

提交于 Nov 30 2016

Gracias por contactarnos.

Tenemos en el catálogo, como bien indicas, tres anticuerpos contra Osteocalcina testados en humano mediante inmunocitoquímica e immunohistoquímica: ab13418, ab13420 y ab13421.

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The AMELX protein ab139212 can be diluted in any buffer. However, all of our products are intended for research use only and are not recommended for in vitro studies. This protein conta...

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Die Lieferung über das Wochenende erfolgt bei uns regelmaessig, ist also kein Grund zur Beunruhigung, da wir erstens den Antikörper mit Kuhlakkus versenden, zweitens FedEx die Produkte im Kühlh...

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I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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I am sorry to hear about the shortage and sincerely apologize about that.

Your free of charge replacement for ab13420 will be shipped with your next order. The free replacement is shipping at full...

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The osteocalcin antibody ab13420 is provided at a concentration of 2 mg/mL, and we recommend using the antibody at 10 ug/mL in IHC-P. So, the antibody will need to be diluted 1:200 bef...

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Expiration date: 21-09-2012

I am very pleased to hear you would like to accept our offer and test ab13420 in [GUNIEA PIG]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before...

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Nous somme vraiment désolés de ne pas avoir pu vous fournir un bon anticorps anti-osteocalcine. J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN est ******.

Voici les mani...

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