antigen: SSAAEPVIVS RSDPRDEKLA LYLAEVEKQD KYLRQRNKYR FHIIPDGNCL YRAVSKTVYG DQSLHRELRE QTVHYIADHL DHFSPLIEGD VGEFIIAAAQ D, corresponding to internal sequence amino acids 272-372 of Human OTUD1 (Q5VV17).
Human testis tissue. RT-4 cell lysate. Human plasma lysate. Human tonsil and liver tissue lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 1 OTU domain.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-OTUD1 antibody (ab122481)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoplasm. Recommended concentration of ab122481 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTUD1 antibody (ab122481)
ab122481 at 1/20 dilution staining OTUD1 in paraffin-embedded Human testis tissue by Immunohistochemistry.
Western blot - Anti-OTUD1 antibody (ab122481)
All lanes : Anti-OTUD1 antibody (ab122481) at 1/250 dilution Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human plasma lysate Lane 4 : Human liver tissue lysate Lane 5 : Human tonsil tissue lysate Developed using the ECL technique.
This product has been referenced in:
et al. Induction of OTUD1 by RNA viruses potently inhibits innate immune responses by promoting degradation of the MAVS/TRAF3/TRAF6 signalosome. PLoS Pathog 14:e1007067 (2018).
Read more (PubMed: 29734366) »
et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016).
Read more (PubMed: 26934049) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"