Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEF)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
5 µg
Specification
MEF
Blocking step
Blocking One (nacalai tesque) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: HIKARI (nacalai tesque)
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: DAKO
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime with C-Digit Chemiluminescent
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 62 kDa
Positive control
all samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
教郎 鈴木
Verified customer
提交于 Dec 17 2013