Native小鼠Fibrinogen蛋白(Active) (ab92791)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
-
产品名称
Native小鼠Fibrinogen蛋白(Active)
参阅全部 Fibrinogen 蛋白酶 -
生物活性
>80% Clottable in a thrombin based assay.
-
纯度
> 95 % SDS-PAGE.
Prepared from fresh Mouse plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. -
表达系统
Native -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
-
氨基酸序列 1
-
序列
MLSLRVTCLI LSVASTVWTT DTEDKGEFLS EGGGVRGPRV VERHQSQCKD SDWPFCSDDD WNHKCPSGCR MKGLIDEANQ DFTNRINKLK NSLFDFQRNN KDSNSLTRNI MEYLRGDFAN ANNFDNTYGQ VSEDLRRRIE ILRRKVIEKA QQIQALQSNV RAQLIDMKRL EVDIDIKIRS CKGSCSRAVN REINLQDYEG HQKQLQQVIA KELLPTKDRQ YLPALKMSPV PDLVPGSFKS QLQEAPPEWK ALTEMRQMRM ELERPGKDGG SRGDSPGDSR GDSRGDFATR GPGSKAENPT NPGPGGSGYW RPGNSGSGSD GNRNPGTTGS DGTGDWGTGS PRPGSDSGNF RPANPNWGVF SEFGDSSSPA TRKEYHTGKA VTSKGDKELL IGKEKVTSSG TSTTHRSCSK TITKTVTGPD GRREVVKEVI TSDDGSDCGD ATELDISHSF SGSLDELSER HPDLSGFFDN HFGLISPNFK EFGSKTHSDS DILTNIEDPS SHVPEFSSSS KTSTVKKQVT KTYKMADEAG SEAHREGETR NTKRGRARAR PTRDCDDVLQ TQTSGAQNGI FSIKPPGSSK VFSVYCDQET SLGGWLLIQQ RMDGSLNFNR TWQDYKRGFG SLNDKGEGEF WLGNDYLHLL TLRGSVLRVE LEDWAGKEAY AEYHFRVGSE AEGYALQVSS YRGTAGDALV QGSVEEGTEY TSHSNMQFST FDRDADQWEE NCAEVYGGGW WYNSCQAANL NGIYYPGGTY DPRNNSPYEI ENGVVWVPFR GADYSLRAVR MKIRPLVGQ -
氨基酸
1 to 789 -
额外的序列信息
Alpha chain Gene ID: 14161
-
氨基酸序列 2
-
序列
MRHLWLLLLL CVFSVQTQAA DDDYDEPTDS LDARGHRPVD RRKEEPPSLR PAPPPISGGG YRARPAKATA NQKKVERRPP DAGGCLHADT DMGVLCPTGC TLQQTLLNQE RPIKSSIAEL NNNIQSVSDT SSVTFQYLTL LKDMWKKKQA QVKENENVIN EYSSILEDQR LYIDETVNDN IPLNLRVLRS ILEDLRSKIQ KLESDISAQM EYCRTPCTVS CNIPVVSGKE CEEIIRKGGE TSEMYLIQPD TSIKPYRVYC DMKTENGGWT VIQNRQDGSV DFGRKWDPYK KGFGNIATNE DAKKYCGLPG EYWLGNDKIS QLTRMGPTEL LIEMEDWKGD KVKAHYGGFT VQNEASKYQV SVNKYKGTAG NALMDGASQL VGENRTMTIH NGMFFSTYDR DNDGWVTTDP RKQCSKEDGG GWWYNRCHAA NPNGRYYWGG LYSWDMSKHG TDDGVVWMNW KGSWYSMRRM SMKIRPFFPQ Q -
氨基酸
1 to 481 -
额外的序列信息
Gene ID: 110135 Beta chain.
-
氨基酸序列 3
-
序列
MSWSLQPPSF LLCCLLLLFS PTGLAYVATR DNCCILDERF GSFCPTTCGI ADFLSSYQTD VDNDLRTLED ILFRAENRTT EAKELIKAIQ VYYNPDQPPK PGMIDSATQK SKKMVEEIVK YEALLLTHET SIRYLQEIYN SNNQKITNLK QKVAQLEAQC QEPCKDSVQI HDTTGKDCQE IANKGAKESG LYFIRPLKAK QQFLVYCEID GSGNGWTVLQ KRIDGSLDFK KNWIQYKEGF GHLSPTGTTE FWLGNEKIHL ISMQSTIPYA LRIQLKDWNG RTSTADYAMF RVGPESDKYR LTYAYFIGGD AGDAFDGYDF GDDPSDKFFT SHNGMQFSTW DNDNDKFEGN CAEQDGSGWW MNKCHAGHLN GVYHQGGTYS KSSTTNGFDD GIIWATWKSR WYSMKETTMK IIPFNRLSIG EGQQHHMGGS KQAGDV -
氨基酸
1 to 436 -
额外的序列信息
Gene ID: 99571 Gamma chain.
-
-
描述
Native Fibrinogen蛋白(Active)
相关产品
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab92791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
Functional Studies
-
形式
Liquid -
补充说明
Extinction coefficient: 1.51
Host species: Mouse
MW: 340.00 kDa
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.40
Constituent: 0.59% Sodium citrateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- FGA
- FGB
- FGG
see all -
功能
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
组织特异性
Plasma. -
疾病相关
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
序列相似性
Contains 1 fibrinogen C-terminal domain. -
结构域
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
翻译后修饰
The alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
细胞定位
Secreted. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (2)
ab92791 被引用在 2 文献中.
- Jansen LE et al. Zwitterionic PEG-PC Hydrogels Modulate the Foreign Body Response in a Modulus-Dependent Manner. Biomacromolecules 19:2880-2888 (2018). PubMed: 29698603
- Waffarn EE et al. Infection-induced type I interferons activate CD11b on B-1 cells for subsequent lymph node accumulation. Nat Commun 6:8991 (2015). PubMed: 26612263