Western blot Abreview for LMW Kininogen protein (Human)

Good
Abreviews
Application
Western blot

Sample: Human Purified protein (Purified LMW Kininogen diluted in Laemmli buffer)
Loading amount: 0.01 µg
Specification: Purified LMW Kininogen diluted in Laemmli buffer
Gel Running Conditions: Reduced Denaturing (12%)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details
Incubation time: 15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non fat milk

Primary antibody (in addition to 'LMW Kininogen protein (Human)')
Dilution: 1/100

Secondary antibody
Name: Non-Abcam antibody was used: goat anti-rabbit IgG-HRP,Santa Cruz biotechnology
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/1000

Detection
Detection method: Thermo scientific super-signal west pico
Exposure: 20 minute(s) and 0 second(s)
Bands: Specific: 68 kDa
Positive control: Coomassie Blue Staining of 1D gel was performed prior WB application

Additional data
Additional Notes: Western blot - LMW Kininogen purified protein ab91118. The blot is stained with ab79650, Anti LMW Kininogen antibody, rabbit polyclonal.
Coomasie Blue stain - LMW Kininogen purified protein ab91118.

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提交于 Jun 29 2012

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