存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituent: 2.68% PBS
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab69954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
相关性NUPL1 is a member of the nucleoporin family. It is a component of the nuclear pore complex, a complex required for trafficking across the nuclear membrane.
细胞定位Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Biased towards cytoplasmic side. Central region of the nuclear pore complex, within the transporter.
- KIAA0410 antibody
- Nucleoporin like 1 antibody
- Nucleoporin p58/p45 antibody
All lanes : Anti-NUPL1 antibody (ab69954) at 1/500 dilution
Lane 1 : NUPL1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
ab69954 at 10µg/ml staining NUPL1 in Hela cells by Immunocytochemistry/Immunofluorescence.
ab69954 has not yet been referenced specifically in any publications.