Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryo)
Specification
embryo
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6%
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594 chicken anti-goat antibody
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
The antibody really gives poor detection if there is any.
So... You might be able make it work, if you try really hard.
So... You might be able make it work, if you try really hard.
Abcam response
Thank you for your feedback. You have already contacted our technical support team regarding this issue and were offered ab14140 as replacement vial as we believe the vial you received was damaged during shipping. You have also recently informed us you are happy with the replacement vial and we look forward to receiving your review.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 13 2007