Application
ChIP
Sample
Mouse Cell lysate - nuclear (Primary CD4+ Th1 cells, non activated or activated)
Negative control
Insulin gene (not active in T cells) (Primer Ins_neg).
Specification
Primary CD4+ Th1 cells, non activated or activated
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
Known p50 target site at the transcriptional start site of NFkB2 (Primer: NFkB2_TSS_1).
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 5°C · Diluent: Chip IP buffer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Kathrin Davari
Verified customer
提交于 Sep 27 2016