Application
ChIP
Sample
Rabbit Cell lysate - nuclear (Primary mouse CD4+ Th1 Cells, non activated and ac)
Negative control
Insulin gene (inactive in T cells) (Primer Ins neg).
Specification
Primary mouse CD4+ Th1 Cells, non activated and ac
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
Known p50 binding site at the transcriptional start site of NFkB2 (Primer NFkB2_TSS_1) and in the Bcl3 intron (Primer Bcl3 Intr_2).
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Chip IP buffer
Additional data
Additional Notes
Insulin gene (inactive in T cells) (Primer Ins neg) used as negative control in ChIP.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Kathrin Davari
Verified customer
提交于 Sep 28 2016