Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s)
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Vector Biotinylated Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Staining may be more optimal with higher concentration of primary antibody. DAB (brown) shows sites of antigen and blue is hematoxylin counterstain.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Simon Cleary
Verified customer
提交于 May 09 2016