Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (WGCTITALEV KDRQGRASDV VLGFAELEGY LQKQPYFGAV IGRVANRIAK) of Human Mutarotase (NP_620156).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/62500.
||Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.
Carbohydrate metabolism; hexose metabolism.
Belongs to the aldose epimerase family.
Information by UniProt
- Aldose 1 epimerase antibody
- Aldose 1-epimerase antibody
- BLOCK25 protein antibody
Western blot - Anti-Mutarotase antibody (ab83238)
Anti-Mutarotase antibody (ab83238) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
38 kDaObserved band size:
34 kDa (why is the actual band size different from the predicted?
Gel concentration 12%
has not yet been referenced specifically in any publications.
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