Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Mouse Cell lysate - whole cell (Mouse breast cancer cell line)
Specification
Mouse breast cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Tween 20 + 5% BSA
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody (Cell Signlain
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 75 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
Lane 2: Mouse breast cancer cells transfected with knockdown shRNA targeting Mre11 gene
Positive control
Lane 1: Mouse breast cancer cells transfected with vector control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 15 2013