Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
10 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 27°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~97 kDa
Positive control
HeLa cell lysate
Negative control
Mps1 treated HeLa cell lysates
Additional data
Additional Notes
Lane 1: HeLa cell lysate
Lane 2: Mps1 siRNA treated HeLa cell lysate as specificity control
Bub3 as loading control
Lane 2: Mps1 siRNA treated HeLa cell lysate as specificity control
Bub3 as loading control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 23 2009