产品名称小鼠and兔Specific HRP Plus (ABC) Detection IHC试剂盒(Ready To Use)
参阅全部 Polyvalent HRP 试剂盒
The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
Reacts with Mouse and Rabbit IgG (H+L)
经测试应用适用于: IHC-Pmore details
存放说明Store at +4°C. Please refer to protocols.
组件 60 ml 125 ml 60 ml 125 ml Biotinylated Goat Anti-Polyvalent Plus 1 x 60ml 1 x 125ml 1 x 60ml 1 x 125ml Protein block 1 x 60ml 1 x 125ml 1 x 60ml 1 x 125ml Streptavidin Peroxidase Plus 1 x 60ml 1 x 125ml 1 x 60ml 1 x 125ml
相关性Polyvalent HRP is a labeled streptavidin-biotin immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates.
Our Abpromise guarantee covers the use of ab93697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
Immunohistochemistry analysis of mouse femur tissue sections. Femurs were fixed in 10% neutral buffered formalin and decalcified for 7 days in 15% EDTA; complete decalcification was confirmed by X-ray. Decalcified bones were embedded in paraffin and sectioned longitudinally through the defect at a thickness of 8 microns. Immunohistochemical staining was performed with monoclonal anti-activated beta-catenin (un-phosphorylated on Ser33/37 and Thr41), or an IgG isotype control. Chromogens were developed using ab93697, Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready to Use), followed by 3′-3′-diaminobenzidine (DAB), and then counterstained with fast green. Beta-catenin levels are elevated in defects of Sost-/- mice.
This product has been referenced in:
- Sabzevary-Ghahfarokhi M et al. The regulatory role of Nrf2 in antioxidants phase2 enzymes and IL-17A expression in patients with ulcerative colitis. Pathol Res Pract 214:1149-1155 (2018). Read more (PubMed: 29941222) »
- Al Asmari AK & Khan AQ Investigation of in vivo potential of scorpion venom against skin tumorigenesis in mice via targeting markers associated with cancer development. Drug Des Devel Ther 10:3387-3397 (2016). IHC ; Mouse . Read more (PubMed: 27799739) »