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|Serum||97||92% - 102%|
|Tissue Culture Media||95||94% - 96%|
|Heparin Plasma||81||77% - 84%|
|EDTA Plasma||104||96% - 111%|
|Citrate Plasma||101||95% - 107%|
Abcam’s IGFBP2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IGFBP2 protein in mouse serum, plasma, and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Insulin-like growth factor-binding protein 2 (IGFBP2) is an IGF binding protein involved in prolonging its half-life, and has both inhibitory and stimulatory effects on IGFs. IGFBP2 can also alter the interaction of IGFs with their receptors.
|组件||1 x 96 tests|
|10X Mouse IGFBP2 Capture Antibody||1 x 600µl|
|10X Mouse IGFBP2 Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent 4BR||1 x 6ml|
|Mouse IGFBP2 Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab207615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1.25%, plasma (citrate) 1%, plasma (heparin) 1% and plasma (EDTA) 1:160. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 492.8 ng/mL in serum, 325.6 ng/mL in plasma (citrate), 428.3 ng/mL in plasma (heparin) and 1247.2 ng/mL in plasma (EDTA).
The concentrations of IGFBP2 in rat serum were measured in duplicates, interpolated from the mouse IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are serum 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 357.6 ng/mL in rat serum.
ab207615 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"