小鼠Albumin ELISA试剂盒(ab108791)
Key features and details
- Sensitivity: 1.4 µg/ml
- Range: 1.563 µg/ml - 100 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mouse
概述
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产品名称
小鼠Albumin ELISA试剂盒
参阅全部 Albumin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.2% 批次间 样品 n Mean SD CV% Overall 9.6% -
样品类型
Serum, Plasma -
检测类型
Competitive -
灵敏度
= 1.4 µg/ml -
范围
1.563 µg/ml - 100 µg/ml -
回收率
= 96 %
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检测时间
2h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse
预测可用于: Rat -
产品概述
Mouse Albumin ELISA kit (ab108791) is a competitive ELISA designed for the quantitative measurement of mouse Albumin levels in plasma and serum.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with PBS or TBS buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 2X Biotinylated Mouse Albumin (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Albumin Microplate (12 x 8 wells) 1 test Albumin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
组织特异性
Plasma. -
疾病相关
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
细胞定位
Secreted. - Information by UniProt
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别名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
数据库链接
- Entrez Gene: 11657 Mouse
- Entrez Gene: 24186 Rat
- SwissProt: P07724 Mouse
- SwissProt: P02770 Rat
- Unigene: 16773 Mouse
- Unigene: 202968 Rat
图片
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Mouse Albumin ELISA Kit example standard curve – data provided for demonstration purposes only.
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Albumin measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
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Albumin measured in biological fluids, background signal subtracted (duplicates +/- SD).
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab108791 被引用在 16 文献中.
- Chen J et al. Diabetic kidney disease-predisposing proinflammatory and profibrotic genes identified by weighted gene co-expression network analysis (WGCNA). J Cell Biochem 123:481-492 (2022). PubMed: 34908186
- Tsujinaka H et al. Sustained treatment of retinal vascular diseases with self-aggregating sunitinib microparticles. Nat Commun 11:694 (2020). PubMed: 32019921
- Wen S et al. Caspase-3 Promotes Diabetic Kidney Disease Through Gasdermin E-Mediated Progression to Secondary Necrosis During Apoptosis. Diabetes Metab Syndr Obes 13:313-323 (2020). PubMed: 32104028
- Lorenc VE et al. Hepatocyte growth factor is upregulated in ischemic retina and contributes to retinal vascular leakage and neovascularization. FASEB Bioadv 2:219-233 (2020). PubMed: 32259049
- Hoffman JB et al. Prebiotic inulin consumption reduces dioxin-like PCB 126-mediated hepatotoxicity and gut dysbiosis in hyperlipidemic Ldlr deficient mice. Environ Pollut 261:114183 (2020). PubMed: 32105967