概述

  • 产品名称
    小鼠Albumin ELISA试剂盒
    参阅全部 Albumin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.4%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 样品类型
    Serum, Plasma, Tissue Lysate
  • 检测类型
    Competitive
  • 灵敏度
    = 0.09 µg/ml
  • 范围
    0.098 µg/ml - 25 µg/ml
  • 回收率

    98 %

  • 检测时间
    2h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 产品概述

    Mouse Albumin in vitro competitive ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of mouse Albumin levels in plasma and serum.


    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with PBS or TBS buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

  • 经测试应用
    适用于: Competitive ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

图片

  • Albumin measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
  • Albumin measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve Using ab108791.

实验方案

文献

This product has been referenced in:
  • Fortmann SD  et al. Mousetap, a Novel Technique to Collect Uncontaminated Vitreous or Aqueous and Expand Usefulness of Mouse Models. Sci Rep 8:6371 (2018). Read more (PubMed: 29686307) »
See 1 Publication for this product

客户评价及客户问答

Answer

This ELISA kit was originally optimized for serum and plasma but our lab recently validated it for use with lysates. This is why there is not a lysis buffer included with the kit but you can use RIPA buffer.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册