Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (Expression of Human Mineralocorticoid receptor in)
Specification
Expression of Human Mineralocorticoid receptor in
Fixative
Formaldehyde
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS + 3% Dried Milk + 0.1 % Triton X-100
Secondary antibody
Name
Non-Abcam antibody was used: goat anti mouse FITC
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/400
Additional data
Additional Notes
The figure clearly shows the nuclear localization of MR in CHO K1 cells expressing human MR. The second picture shows a DAPI nuclear stain of the same cells to highlight the nucleus.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 29 2008