Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab94674)
Permeabilization
Yes - 0.05% tween 20
Specification
Breast
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/100
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Thermo #A11034 Goat anti-rabbit Conjugation: Alex
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
10% Neutral Buffered Formalin
Biocare Medical Decloaking Chamber
Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab94674)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. David Ivancic
Verified customer
提交于 May 23 2017