Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (murine melanoma metastasis)
Specification
murine melanoma metastasis
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Other product details
Dilution
1/50
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TNB
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/250
Additional data
Additional Notes
Confocal image shows melanoma in magenta, vessels in red (Collagen Type IV, ab19808), and melanin in cyan. Tissue is perfusion-fixed 15µm cryostat sections mounted on slides (i.e., lightly fixed, but not paraffin embedded). This is the only commercial ab I could really find that would react with mouse melanoma, in this case, B16F10. 4 stars because even at 1:50, I don't get very intense signals. This may improve with frozen sections although I have not tried this yet.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 07 2008