Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Mammary gland)
Specification
Mammary gland
Fixative
Neutral Buffered Formalin
Antigen retrieval step
Other - Buffer/Enzyme Used: Sodium Citrate, EDTA
Permeabilization
Yes - Prot K
Blocking step
Dual endogenous, Protein Block as blocking agent for 10 minute(s) · Concentration: 100%
Other product details
Dilution
1/10
Incubation time
1 hour(s) and 0 minute(s) · Diluent: Dako antibody diluent
Secondary antibody
Name
Non-Abcam antibody was used: Dako Envision Plus
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
No staining was observed in involuting FFPE human mammary tissue where we have shown that mannose receptor positive cells are abundant. Staining was observed with frozen involuting human mammary tissue (see our other Abreview).
Abcam response
Thank you for your feedback. This antibody has not yet been tested in IHC-P only IHC-Fr. You may have to work to optimize it in IHC-P.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 13 2009