参阅全部 MTA1 一抗
经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
种属反应性与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis, Platypus
A synthetic peptide corresponding to a region between residue 665 and the C-terminus (residue 715) of MTA1 (NP_004680.1).
- Whole cell lysate from Hela cells, NIH3T3 cells and 293T cells.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab71153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 35, 81 kDa (predicted molecular weight: 81 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
功能May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER.
组织特异性Widely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual.
序列相似性Contains 1 BAH domain.
Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain.
发展阶段Highly expressed in metastatic cells.
结构域Isoform Short contains a Leu-Arg-Ile-Leu-Leu motif (ER binding motif).
细胞定位Cytoplasm and Nucleus.
- Information by UniProt
- Metastasis associated 1 antibody
- Metastasis associated gene 1 antibody
- Metastasis associated gene 1 protein antibody
All lanes : Anti-MTA1 antibody (ab71153) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 81 kDa
Observed band size: 81 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling MTA1 with ab71153 at 1/1000 (0.2 µg/ml). Detection: DAB.
Immunoprecipitation/ Western Blot of MTA1
Lane 1: ab71153 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Subsequent WB detection was performed using 1 µg/ml ab71153.
Chemiluminescence with an exposure time of 1 second.
ICC/IF image of ab71153 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Patel D et al. The histone demethylase LSD1 regulates inner ear progenitor differentiation through interactions with Pax2 and the NuRD repressor complex. PLoS One 13:e0191689 (2018). Read more (PubMed: 29370269) »
- Yu R et al. miR-377 targets CUL4A and regulates metastatic capability in ovarian cancer. Int J Mol Med 41:3147-3156 (2018). Read more (PubMed: 29512715) »