重组Anti-MMP3抗体[EP1186Y] (ab52915)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1186Y] to MMP3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MMP3抗体[EP1186Y]
参阅全部 MMP3 一抗 -
描述
兔单克隆抗体[EP1186Y] to MMP3 -
宿主
Rabbit -
特异性
79% identities with MMP10 -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human MMP3 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: Raji, HEK293; mouse lung, placenta, brain, kidney liver lysate; rat brain, liver, spleen, kidney lysate; IHC-P: Rat kidney, mouse liver, human liver. ICC/IF: HT-29 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1186Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52915于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
|
IHC-P | (3) |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
We recommend using a polymer-HRP conjugated secondary antibody. Incubate with ab52915 at 4C overnight. |
ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. We recommend using a polymer-HRP conjugated secondary antibody. Incubate with ab52915 at 4C overnight. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4314 Human
- Entrez Gene: 17392 Mouse
- Entrez Gene: 171045 Rat
- Omim: 185250 Human
- SwissProt: P08254 Human
- SwissProt: P28862 Mouse
- SwissProt: P03957 Rat
- Unigene: 375129 Human
see all -
别名
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase 3 preproprotein antibody
see all
图片
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All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte )whole cell lysate
Lane 2 : HEK293 (Human embryonic kidney epithelial cell ) whole cell lysate
Lane 3 : Mouse lung lysate
Lane 4 : Mouse placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 84 secondsBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemical staining of paraffin embedded rat kidney with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescence staining of HT-29 cells with purified ab52915 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52915 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)
Lane 1 : Rat brain lysate
Lane 2 : Rat liver lysate
Lane 3 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1 & 2: 10 seconds
Lane 3: 3 minutes -
All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes
Lane 2 & 3: 4 seconds -
Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified) + Rat kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer: 5% NFDM/TBST
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Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/20000 dilution (purified) + MMP3 recombinant protein
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunohistochemical staining of paraffin embedded mouse liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded human liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (192)
ab52915 被引用在 192 文献中.
- Ono K et al. Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-Free Fractions, and Cells. Methods Mol Biol 2582:39-57 (2023). PubMed: 36370343
- Eguchi T et al. Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Mechanism. Methods Mol Biol 2582:103-126 (2023). PubMed: 36370347
- Takahata K et al. Joint instability causes catabolic enzyme production in chondrocytes prior to synovial cells in novel non-invasive ACL ruptured mouse model. Osteoarthritis Cartilage 31:576-587 (2023). PubMed: 36528308
- Wagner JM et al. Inhibition of Pathological Increased Matrix Metalloproteinase (MMP) Activity for Improvement of Bone Regeneration in Diabetes. Life (Basel) 12:N/A (2022). PubMed: 35207422
- Schick B et al. Neural Crest Stem Cells in Juvenile Angiofibromas. Int J Mol Sci 23:N/A (2022). PubMed: 35216046