Anti-MIA3/TANGO1抗体(ab97881)
Key features and details
- Rabbit polyclonal to MIA3/TANGO1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MIA3/TANGO1抗体
参阅全部 MIA3/TANGO1 一抗 -
描述
兔多克隆抗体to MIA3/TANGO1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Cow -
免疫原
Recombinant fragment corresponding to Human MIA3/TANGO1 aa 1-120.
Database link: XP_496436 -
阳性对照
- A431, H1299, HeLa and HepG2 cell lines
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97881于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 214 kDa.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 214 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. -
组织特异性
Broadly expressed, except in bone marrow and peripheral blood mononuclear cells. Down-regulated in melanoma tissue. -
序列相似性
Belongs to the MIA/OTOR family. Tango1 subfamily.
Contains 1 SH3 domain. -
结构域
The proline-rich region (PRD) mediates the interaction with COPII coat subunits Sec23/24.
Although 2 transmembrane domains are predicted, PubMed:19269366 showed that it only contains one transmembrane domain. The other predicted transmembrane region is probably a hairpin-type region embedded into the membrane, which does not cross the membrane. It is unclear which of the 2 predicted transmembrane regions is the transmembrane or the hairpin-type region. -
细胞定位
Endoplasmic reticulum membrane. Localizes at endoplasmic reticulum exit sites. After loading of COL7A1 into transport carriers, it is not incorporated into COPII carriers and remains in the endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 375056 Human
- Omim: 613455 Human
- SwissProt: Q5JRA6 Human
- Unigene: 118474 Human
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别名
- ARNT antibody
- C219 reactive peptide antibody
- C219-reactive peptide antibody
see all
图片
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All lanes : Anti-MIA3/TANGO1 antibody (ab97881) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : Hep G2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 214 kDa5% SDS PAGE.
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Immunofluorescence analysis of methanol fixed HepG2 cells, using ab97881 at a 1/200 dilution.
Lower image: costained with Hoechst 33342.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab97881 被引用在 2 文献中.
- Du Q et al. Exosomal miR-30a and miR-222 derived from colon cancer mesenchymal stem cells promote the tumorigenicity of colon cancer through targeting MIA3. J Gastrointest Oncol 12:52-68 (2021). PubMed: 33708424
- Abou-Antoun TJ et al. Molecular and functional analysis of anchorage independent, treatment-evasive neuroblastoma tumorspheres with enhanced malignant properties: A possible explanation for radio-therapy resistance. PLoS One 13:e0189711 (2018). PubMed: 29298329