Application
Western blot
Sample
Human Cell lysate - whole cell (T lymphocyte)
Gel Running Conditions
Non-reduced Denaturing (8%)
Loading amount
30 µg
Specification
T lymphocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Human
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Human
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Jurkat T cells
Exposure
15 minute(s) and 0 second(s)
Positive control
Jurkat T cells
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 10 2014