Anti-MGC4618抗体(ab49189)

概述

  • 产品名称
    Anti-MGC4618抗体
    参阅全部 MGC4618 一抗
  • 描述
    兔多克隆抗体to MGC4618
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cat, Dog
  • 免疫原

    A region within synthetic peptide: MLSFSDALLS IIATVMILPV THTEISPEQQ FDRSVQRLLA TRIAVYLMTF, corresponding to N terminal amino acids 36-85 of Human MGC4618

  • 阳性对照
    • HepG2 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:1562500.

靶标

图片

  • Anti-MGC4618 antibody (ab49189) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    Diluted in 5% skim milk / PBS buffer, gel concentration 12%

实验方案

文献

ab49189 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Adult brain)
Gel Running Conditions
Reduced Denaturing (4-15% BioRad Precast)
Loading amount
20 µg
Specification
Adult brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Sep 19 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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